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Organization and evolution of the human growth hormone receptor gene 5-flanking region

Strategy 3: Use the accelerated titration approach

Production of PIP 3 enables phosphorylation of Akt at threonine 308 (T308) by 3-phosphoinositide-dependent protein kinase-1 (PDK1) and at serine 473 (S473) by the mechanistic target of rapamycin complex 2 (mTORC2)

As FOXO4 is a transcription factor, this nuclear localization indicates higher transcriptional regulatory activity, suggesting FOXO4 activity is involved in Leydig cells senescence